[ FreeCourseWeb.com ] Golden Gate Cloning: Methods and Protocols
If You Need More Stuff, kindly Visit and Support Us -->> https://FreeCourseWeb.com
English | 2025 | ISBN: 1071640941 | 500 Pages | EPUB PDF (True) | 78 MB
This volume provides an overview of the latest protocols that cover the full range of in silico, in vitro, and in vivo approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Download from free file storage
If You Need More Stuff, kindly Visit and Support Us -->> https://DevCourseWeb.com
Get Latest Tips and Tricks and Support Us -->> https://FreeCourseWeb.com
We upload these learning materials for the people from all over the world, who have the talent and motivation to sharpen their skills/ knowledge but do not have the financial support to afford the materials. If you like this content and if you are truly in a position that you can actually buy the materials, then Please, we repeat, Please, Support Authors. They Deserve it! Because always remember, without "Them", you and we won't be here having this conversation. Think about it! Peace...