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This second edition volume expands on the previous edition with updated protocols on basic techniques of radioligand binding, allosteric modulation, visualization and antibody use, and autoradiography and non-neuronal acetylcholine detection. The updated chapters also cover the latest fluorescent techniques including fluorescence anisotropy, and provide updates on current system measurements of heart contractility and gastrointestinal tract motility to provide readers with a comprehensive picture of current muscarinic receptors from molecular to systemic levels. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and authoritative, Muscarinic Receptors – From Structure to Animal Models, Second Edition is a valuable resource for scientists who are interested in using the latest methodologies to study muscarinic receptors quickly, easily, and correctly.
Preface to the Series
Preface
Contributors
Radioligand Binding at Muscarinic Receptors
Tissue-Segment Binding Method for Detection of Muscarinic Acetylcholine Receptors in Receptor´s Natural Environment
Use of Antibodies in the Research on Muscarinic Receptor Subtypes
Allosteric Modulation of Ligand Binding to Muscarinic Receptors
Allosteric Modulation of Functional Response of Muscarinic Receptors
Exploring Muscarinic Acetylcholine Receptor Binding Kinetics with Fluorescence Anisotropy
MultiBacMam Technology for Studying the Downstream cAMP Signaling Pathway of M2 Muscarinic Acetylcholine Receptor
Subcellular and Synaptic Distribution of Muscarinic Receptors in Neurons by Confocal and Electron Microscopy
Investigation of Muscarinic Receptors by Fluorescent Techniques
Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System with a Special Focus on the Selec...
Detection of Non-neuronal Acetylcholine
Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release
Evaluation of Acetylcholine Synthesis and Release in Striatal Cholinergic Interneurons
Regulation of Heart Contractility by M2 and M3 Muscarinic Receptors: Functional Studies Using Muscarinic Receptor...
Muscarinic Regulation of Gastrointestinal Motility
Systems for a Long-Term Record of Animal Activity, Temperature, and Heart Rate Affected by Muscarinic Receptors
Index