Torrent details for "Saunders T. Transgenesis. Methods and Protocols 2023 [andryold1]"    Log in to bookmark

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This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.
Transgenesis and Genome Engineering:​ A Historical Review
Practical Application of the 3Rs in Rodent Transgenesis
Genetic and Molecular Quality Control of Genetically Engineered Mice
Genotyping Genome-Edited Founders and Subsequent Generation
ASIS-Seq:​ Transgene Insertion Site Mapping by Nanopore Adaptive Sampling
High-Throughput Analysis of CRISPR-Cas9 Editing Outcomes in Cell and Animal Models Using CRIS.​py
Advanced Technologies and Automation in mES Cell Workflow
Gene Editing in Mouse Zygotes Using the CRISPR/​Cas9 System
Floxing by Electroporating Single-Cell Embryos with Two CRISPR RNPs and Two ssODNs
CRISPR/​Cas9 Endonuclease-Mediated Mouse Genome Editing of One-Cell and/​or Two-Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Interhomolog Repair Mechanism
Electroporation-Mediated CRISPR/​Cas9 Genome Editing in Rat Zygotes
CRISMERE Chromosome Engineering in Mouse and Rat
Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus
Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes
Gene Targeting in Rat Embryonic Stem Cells
Rat Embryonic Stem Cell Transgenesis
CRISPR/​Cas9-Mediated Genome Editing in Zebrafish
Generation of Rabbit Chimeras by Eight-Cell Stage Embryo Injection
Genome Editing in Pigs
Generation of Genome-Edited Chicken Through Targeting of Primordial Germ Cells

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