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This detailed volume presents cutting-edge research protocols to study the structure and dynamics of bacterial and archaeal motility systems using bacterial genetics, molecular biology, biochemistry, biophysics, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a section on bacterial flagellar protein export and assembly, the book continues with chapters covering flagella-driven motility of bacteria, archaella-driven motility of archaea, type IV-driven twitching motility of bacteria, as well as adhesion-based gliding motility of bacteria and other unique motility systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Bacterial and Archaeal Motility is the ideal reference for researchers working in this vital area of microbiology.
Bacterial Flagellar Protein Export and Assembly
Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System
In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles
Molecular Simulation to Investigate Open–Close Motion of a Flagellar Export Apparatus Protein FlhAC
Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy
Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament
Flagella-Driven Motility of Bacteria
Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy
Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo
Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor
Purification of the Na -Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy
Purification of Na -Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy
High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook
Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility
Swarming Motility Assays in Salmonella
Analysis of Adhesion and Surface Motility of a Spirochete Bacterium
Force Measurement of Bacterial Swimming Using Optical Tweezers
Archaella-Driven Motility of Archaea
Archaella Isolation
Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques
Type IV-Driven Twitching Motility of Bacteria
In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography
Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope
Live Cell Imaging of the Twitching Motility of Cyanobacteria by High-Resolution Microscopy
Adhesion-Based Gliding Motility of Bacteria
Isolation and Visualization of Gliding Motility Machinery in Bacteroidota
Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniae Under High-Resolution Microscopy
Social Motility Assays of Flavobacterium johnsoniae
Visualization of Peptidoglycan Structures of Escherichia coli by Quick-Freeze Deep-Etch Electron Microscopy
Unique Motility System in Bacteria
Purification and Structural Analysis of the Gliding Motility Machinery in Mycoplasma mobile
Motility Assays of Mycoplasma mobile Under Light Microscopy
Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile
Direct Measurement of Kinetic Force Generated by Mycoplasma
Genetic Manipulation of Mycoplasma pneumoniae
Purification and ATPase Activity Measurement of Spiroplasma MreB
Swimming Motility Assays of Spiroplasma
Motility Assays of Chloroflexus