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This open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this book cover topics over a broad range, from fundamental background information in both physiology and optics in the context of all-optical neurophysiology experiments, to the design principles and hardware implementation of optical methods used for photoactivation and imaging. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and cutting-edge, All-Optical Methods to Study Neuronal Function is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors.
Preface
Contributors
Optical Manipulation and Recording of Neural Activity with Wavefront Engineering
Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments
Light-Based Neuronal Circuit Probing in Living Brains at High Resolution: Constraints and Layouts for Integrating N...
High-Speed All-Optical Neural Interfaces with 3D Temporally Focused Holography
An All-Optical Physiology Pipeline Toward Highly Specific and Artifact-Free Circuit Mapping
Spatial and Temporal Considerations of Optogenetic Tools in an All-Optical Single-Beam Experiment
Miniature Multiphoton Microscopes for Recording Neural Activity in Freely Moving Animals
Optogenetics and Light-Sheet Microscopy
Widefield Multiphoton Imaging at Depth with Temporal Focusing
High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectio...
Generation of Annular Illumination with an SLM (Adapted from Ref.)
Generation of Annular Illumination with an Axicon
Calculation of Two-Photon Excitation PSF
Design of the Annular Aperture Mask in an SLM-Based Bessel Module (Adapted from Ref.)
Design of an Axicon-Based Bessel Module
Alignment of Bessel Module (Adapted from Ref.)
Installation of the Optical Components
Gross Alignment of the Bessel and Gaussian Beam Paths
Fine Alignment
Results and Data Analysis
Laser Beam Multiplexing, Shuttering, and Expansion
Beam Modulation Module
Maximizing Diffraction Efficiency and Pattern Contrast at the Sample Plane
Fine Alignment
Refined OS-SIM Reconstruction Method
Fast Functional Imaging Using OS-SIM
Parameter Selection in Image Reconstruction
Other Optical Sectioning Reconstruction Methods
Other Considerations for In Vivo Imaging
Optical and Analytical Methods to Visualize and Manipulate Cortical Ensembles and Behavior
Illuminating Neural Computation Using Precision Optogenetics-Controlled Synthetic Perception
Spectrally Focused Stimulated Raman Scattering (sf-SRS) Microscopy for Label-Free Investigations of Molecular Mech...
Index