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This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.
From Olive Oil Emulsions to COVID-19 Vaccines:​ Liposomes Came First
Preparation of DRV Liposomes
Preparation of Small Unilamellar Vesicle Liposomes Using Detergent Dialysis Method
Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation
Ethanol Injection Method for Liposome Preparation
Preparation of Giant Vesicles with Mixed Single-Tailed and Double-Tailed Lipids
Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing
Preparation of Doxorubicin Liposomes by Remote Loading Method
Magnetic Thermosensitive Liposomes Loaded with Doxorubicin
Preparation and Physical Characterization​ of DNA Binding Cationic Liposomes
Tunable pH Sensitive Lipoplexes
Solid Lipid Nanoparticles for Drug Delivery
Stable Discoidal Bicelles:​ Formulation, Characterization​, and Functions
The Post-insertion Method for the Preparation of PEGylated Liposomes
Click Chemistry for Liposome Surface Modification
Surface Modification of Liposomes Using Folic Acid
Preparation and Characterization​ of Trastuzumab Fab-Conjugated Liposomes (Immunoliposomes)
Pyrophosphorylat​ed-Cholesterol-Modified Bone-Targeting Liposome Formulation Procedure
Method of Simultaneous Analysis of Liposome Components Using HPTLC/​FID
High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry Method for the Identification and Quantification of Lipids in Liposomes
DPH Probe Method for Liposome-Membrane Fluidity Determination
Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy
Visualization and Characterization​ of Liposomes by Atomic Force Microscopy
Determination of the Subcellular Distribution of Fluorescently Labeled Liposomes Using Confocal Microscopy
Liposome Biodistribution via Europium Complexes
Quantification of a Fluorescent Lipid DOPE-NBD by an HPLC Method in Biological Tissue:​ Application to Study Liposomes’ Uptake by Human Placenta