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This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification, epidemiology, and transcriptome analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comprehensive guide for researchers.
Nanopore Sequencing for Genomics and Beyond
The Current State of Nanopore Sequencing
Hybrid Genome Assembly of Short and Long Reads in Galaxy
Microbial Genome Sequencing and Assembly Using Nanopore Sequencers
De Novo Genome Assembly of Japanese Black Cattle as Model of an Economically Relevant Animal
How to Sequence and Assemble Plant Genomes
Detection of DNA Modification Using Nanopore Sequencers
Ultralow-Input Genome Library Preparation for Nanopore Sequencing with Droplet MDA
The Method of Eliminating the Wolbachia Endosymbiont Genomes from Insect Samples Prior to a Long-Read Sequencing
A Nanopore Sequencing Course for Graduate School Curriculum
Analysis of Repetitive Regions and Structural Variants
A Guide to Sequencing for Long Repetitive Regions
Analysis of Tandem Repeat Expansions Using Long DNA Reads
Finding Rearrangements in Nanopore DNA Reads with LAST and dnarrange
Long-Read Whole-Genome Sequencing Using a Nanopore Sequencer and Detection of Structural Variants in Cancer Genomes
Rapid On-Site Microbial Detection and Epidemiology
Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens
Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System
Genomic Epidemiological Analysis of Antimicrobial-Resistant Bacteria with Nanopore Sequencing
Rapid and Comprehensive Identification of Nontuberculous Mycobacteria
Nanopore Sequencing for Transcriptomics and Beyond
Long-Read Single-Cell Sequencing Using scCOLOR-seq
Unfolding the Bacterial Transcriptome Landscape Using Oxford Nanopore Technology Direct RNA Sequencing
Nanopore Direct RNA Sequencing of Monosome- and Polysome-Bound RNA
RNA Modification Detection Using Nanopore Direct RNA Sequencing and nanoDoc2