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This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.
Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis
Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation
Multi-macromolecular Extraction from Endosymbiotic Anthozoans
High-Resolution Liquid Chromatography–Mass Spectrometry for Lipidomics
Characterization​ of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry
Protocol and Methods Applicable to Retinal Vascular Diseases
Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics
Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics
Lipidomics Analysis with Triple Quadrupole Mass Spectrometry
High-Performance Chromatographic Separation of Cerebrosides
Quantification of Endocannabinoids​ from Biological Samples Using Liquid Chromatography–Tandem Mass Spectrometry
Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains
Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry
Analyses and Localization of Phosphatidylchol​ine and Phosphatidylseri​ne in Murine Ocular Tissue Using Imaging Mass Spectrometry
A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets
Capillary Electrophoresis Plasma Fractionation for Lipids Analysis
Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for the Analysis of (Phospho)Lipids
Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles (OMVs) of Pseudomonas Aeruginosa
Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells
Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate
Lipid Extraction and Sample Preservation Techniques for Stable Isotope Analysis and Ecological Assays
Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry
Untargeted Analysis of Lipids Containing Very Long Chain Fatty Acids in Retina and Retinal Tight Junctions
Analysis of Lipid Contents in Human Trabecular Meshwork Cells by Multiple Reaction Monitoring (MRM) Profiling Lipidomics
Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples
Downloading and Analysis of Metabolomic and Lipidomic Data from Metabolomics Workbench Using MetaboAnalyst 5.​0
Accurate Analysis of Lipid Building Blocks Using the Tool LipidOne
Image-Based Longitudinal Characterization​ of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate
Characterization​ of Trabecular Meshwork Mechanical Property Modulation After Application of Lipids
C-Laurdan:​ Membrane Order Visualization of HEK293t Cells by Confocal Microscopy
Sonication-Based Basic Protocol for Liposome Synthesis