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This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Detection of miRNAs
MicroRNA Identification, Target Prediction, and Validation for Crop Improvement
MicroRNA Detection with CRISPR/​Cas
Detection of MicroRNAs by Northern Blot
MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore
Detection of MicroRNA Expression Dynamics Using LNA/​DNA Nanobiosensor
Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification
Small RNA Profiling by Next-Generation Sequencing Using High-Definition Adapters
Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification
Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine
Determining miRNA Expression Patterns in Xenopus
Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets
sRNAtoolbox:​ Dockerized Analysis of Small RNA Sequencing Data in Model and Non-model Species
MicroRNA–Target Identification:​ A Combinatorial In Silico Approach
An Efficient CRISPR-Cas9 Method to Knock Out MiRNA Expression in Xenopus Tropicalis
Interrogation of Functional miRNA-Target Interactions by CRISPR/​Cas9 Genome Engineering