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This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.
Preface
Technological Innovations
Capillary electrophoresis/mass spectrometry for the determination of bioactive proteins and peptides
Preconcentration techniques in high performance capillary electrophoresis
Capillary electrophoresis with laser-induced fluorescence detection: optical designs and applications
Protein and Peptide Analysis
Analysis of protein folding by capillary electrophoresis
HPCE monitoring of phosphorylated histone HI variants
Application of HPCE-analysis to process monitoring in biotechnology
Isoform characterization, peptide mapping and amino acids analysis of monoclonal antibodies by high performance capillary electrophoresis
Capillary zone electrophoresis and isotachophoresis of biologically active peptides
HPCE in Drug Abuse and Drug Interactions
Analysis of drugs of abuse by micellar electrokinetic capillary chromatography
Study of protein-drug binding by capillary zone electrophoresis: Prospects and problems
HPCE of catecholamines, bioactive molecules and other transmitters
Capillary gel electrophoresis and antisense therapeutics: The analysis of DNA analogs
HPCE of Carbonhydrates, Chiral and Enantiomers
Capillary electrophoresis of heparin-derived carbohydrate chains
Chiral separation by HPCE with oligosaccharides: Application to acidic compounds
Separation of enantiomers in capillary electrophoresis
HPCE of Aniona and Cations
Negative absorbance: Application to the detection of inorganic anions and cations in high performance capillary electrophoresis
Author index
Subject index