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This volume explores multiple methods and approaches used to generate human brain and neuroretinal organoids to address fundamental questions in human brain research. The chapters in this book cover topics such as self-organizing brain organoids with ventricles amenable to injection and electroporation visualization of 3D organoids though the latest advancements in microscopy generation of 3D retinal tissue with physiologically competent, light-sensitive photoreceptors modeling brain tumors using genetically edited brain organoids and brain organoids as a model to study Zika virus and SARS-CoV-2 infections. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Brain Organoid Research is a valuable resource for researchers at various levels of learning, ranging from undergraduate students, early researchers, and advanced laboratories. This book aims to be instrumental in moving this developing field forward.
Self-Organizing Brain Organoids with Ventricles Amenable to Injection and Electroporation
Profiling Cell Type-Specific Gene Regulatory Regions in Human Cortical Organoids
Visualization of 3D Organoids Through the Latest Advancements in Microscopy
Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell Sorting
Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into Mice
Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light-Sensitive Photoreceptors from Human-Induced Pluripotent Stem Cells
Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids
A Three-Dimensional Organoid Culture System to Model Invasive Patterns of Patient-Derived Glioma Stem Cells
Modeling Brain Tumors Using Genetically Edited Brain Organoids
Brain Organoids as a Model to Study Zika Virus and SARS-CoV-2 Infections
Infection of 3D Brain Organoids with Human Pathogenic Viruses Under Biosafety Level-3 Conditions with Subsequent Inactivation to Study Viral Replication, Pathomechanisms, and Other Viral Infection-Mediated Effects