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This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside.
A Fungal Protease Model to Interrogate Allergic Lung Immunity
Use of the Litomosoides sigmodontis Infection Model of Filariasis to Study Type 2 Immunity
Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool with New Clinical Applications
A Mouse Model of Peanut Allergy Induced by Sensitization Through the Gastrointestinal​ Tract
Induction and Characterization​ of the Allergic Eye Disease Mouse Model
Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung
In Vitro and In Vivo IgE-/​Antigen-Mediated Mast Cell Activation
The Use of Human and Mouse Mast Cell and Basophil Cultures to Assess Type 2 Inflammation
Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation
Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors
Characterization​ of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry
Characterization​ of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity
The Identification of Allergen-Derived T Cell Epitopes
Generation of Allergen-Specific Tetramers for a Murine Model of Airway Inflammation
The Generation and Use of Allergen-Specific TCR Transgenic Animals
Using Cytokine Reporter Mice to Visualize Type-2 Immunity In Vivo
Live Imaging of IL-4-Expressing T Follicular Helper Cells in Explanted Lymph Nodes
Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking
Study of IgE-Producing B Cells Using the Verigem Fluorescent Reporter Mouse
Chromatin Preparation from Murine Eosinophils for Genome-Wide Analyses
A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers
An Integrated and Semiautomated Microscaled Approach to Profile Cis -Regulatory Elements by Histone Modification ChIP-Seq for Large-Scale Epigenetic Studies
Library Preparation for ATAC-Sequencing of Mouse CD4  T Cells Isolated from the Lung and Lymph Nodes After Helminth Infection
Identification of Functionally Relevant microRNAs in the Regulation of Allergic Inflammation
The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation
Assessing the Mouse Intestinal Microbiota in Settings of Type-2 Immune Responses
The Use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology
A Consistent Method to Identify and Isolate Mononuclear Phagocytes from Human Lung and Lymph Nodes
Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation
Utilization of Air–Liquid Interface Cultures as an In Vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin-13