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This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.
Evaluation of Prototype CE-MS Interfaces
Capillary ElectrophoresisâMass Spectrometry (CE-MS) by SheathâFlow Nanospray Interface and Its Use in Biopharmaceutical Applications
Protocol for Etching Bare-Fused Silica Capillaries for Sheathless Capillary ElectrophoresisâMass Spectrometry Coupling
Microfabricated Liquid Junction Capillary ElectrophoresisâMass Spectrometry Interface
Successive Multiple Ionic-Polymer Layer Coatings for Intact Protein Analysis by Capillary Zone ElectrophoresisâMass Spectrometry: Application to Hemoglobin Analysis
On-line Immobilized Enzyme Microreactor Capillary Zone ElectrophoresisâMass Spectrometry for Peptide Mapping
Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Tryptic Digest
Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Characterization of Proteoforms in Complex Samples
Assessment of Macro- and Microheterogeneity of Monoclonal Antibodies Using Capillary Zone Electrophoresis Hyphenated with Mass Spectrometry
High-Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS
Capillary Zone ElectrophoresisâElectrospray Ionization Tandem Mass Spectrometry for Total Analysis of Chondroitin/Dermatan Sulfate Oligosaccharides
In-Source Fragmentation for the Identification of Compounds by CE-ESI-TOF in Human Plasma. l-Proline as Case Study
Assessing the Energy Status of Low Numbers of Mammalian Cells by Capillary ElectrophoresisâMass Spectrometry
Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis
Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction Coupled to Capillary ElectrophoresisâTandem Mass Spectrometry
CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis