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This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production.
Recombinant Protein Production in Plants: A Brief Overview of Strengths and Challenges
Plant Production Systems and Applications
Production of Recombinant Proteins in Transgenic Tobacco Plants
Molecular Farming in Seed Crops: Gene Transfertransfer into Barley (Hordeum vulgare ) and Wheat (Triticum aestivum )
Cell Biology Methods to Study Recombinant Proteins in Seeds
Production of Recombinant Glycoproteins in Nicotiana tabacum tabacum BY-2 Suspension Cells
Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression
Specific Packaging of Custom RNA Molecules into Cowpea Mosaic Virus-like Particles
Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins
Downstream Processing
Strategies for Efficient and Sustainable Protein Extraction extractions and Purification extractions from Plant Tissues
Improving Recombinantrecombinant Protein Recoveryrecovery recombinant from Plant Tissue Using Heat Precipitation
Technoeconomic Modeling and Simulation for Plant-Based Manufacturing of Recombinant Proteins
Optimization Strategies
Optimization of Vectors and Targetingtargeting Strategies Including GoldenBraid and Genome Editing Tools: GoldenBraid Assembly of Multiplex CRISPR /Cas12a Guide RNAs for Gene Editing in Nicotiana benthamiana benthamiana
Advanced Fusion Strategies for the Production of Functionalized Potato Virus XPotato virus X Virions
Knockout of Glycosyltransferases in Nicotiana benthamiana benthamiana by Genome Editing to Improve Glycosylation benthamiana of Plant-Produced Proteins
A Bioluminescent Agrobacterium tumefaciens tumefaciens for Imaging Bacterial Metabolic Activity in Planta
Statistical Designs to Improve Downstream Processing
Regulatory Issues
Regulation of Molecular Farming Products
Freedom to Operate Analysis of Molecular Farming Projects