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This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Sulfated Glycoprotein Synthesis
Total Chemical Synthesis of a SARS-CoV-2 Miniprotein Inhibitor LCB1
Serine/​Threonine Ligation and Cysteine/​Penicillamine Ligation
Continuous-Flow Synthesis of N-Methylated Peptides via Generation of an Acyl N-Methylimidazolium Cation
Covalent Reactive Peptides to Block Protein–Protein Interactions and Inhibit Microbe–Host Interaction
Ynamide-Mediated Synthetic Approach to Thioamide-Substituted Peptides
Improved Handling of Peptide Segments Using Side Chain-Based “Helping Hand” Solubilizing Tools
General Solution to the Preparation of β-Thiolated/Selenolated Amino Acids Via Visible Light Catalyzed Asymmetric Giese Reaction
Side-Chain Anchoring Strategies for the Synthesis of Peptide Thioesters and Selenoesters
Preparation of Site-Specific Succinylated Histone Mimics to Investigate Its Impact on Nucleosome Dynamics
Protein Synthesis via Activated Cysteine-Directed Protein Ligation
Sulfonium-Tethered Peptide
PAL-Mediated Ligation for Protein and Cell-Surface Modification
Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation
A Selenium-based Cysteine Surrogate for Protein Chemical Synthesis
Removable Backbone Modification (RBM) Strategy for the Chemical Synthesis of Hydrophobic Peptides/​Proteins