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As in the second edition, the third edition of Handbook of Practical Immunohistochemistry – Frequently Asked Questions is written in a question and answer (Q&ampA) format and intended to be a practical, user-friendly, quick reference for information related to using the most up-to-date immunohistochemistry and in situ hybridization in clinical diagnosis. The new edition demonstrates a significant revision and improvement over the second edition. Five new chapters have been added that cover the following: 1) Immunohistochemistry: Leica’s perspective 2) Immunohistochemistry: Maixin perspective 3) RNA in situ hybridization: Applications in anatomic pathology 4) Applications of rapid immunohistochemistry on frozen tissue sections during intraoperative pathologic diagnosis and 5) Cutaneous lymphomas. In addition to extensive additions and changes, over 150 new questions and answers were added throughout this new edition. All chapters have been updated to include relevant new questions, new markers, more refined IHC panels, representative pictures, and current references. An extensive set of high-quality color pictures and diagnostic algorithms, if available, is included in each chapter to illustrate some of the key antibodies, including many recently discovered and substantiated antibodies used in that chapter. Written by experts in the field, Handbook of Practical Immunohistochemistry Third Edition is a comprehensive and practical guide for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
Immunohistochemi​stry Quality Management and Regulation
Standardization of Diagnostic Immunohistochemi​stry
Automated Immunohistochemi​stry Overview
Immunohistochemi​stry:​ An Agilent Perspective
The Leica Biosystems Perspective:​ From Excision to Imaging—Every Step Is Critical
Immunohistochemi​stry:​ Maixin Perspective
Immunohistochemi​stry:​ Roche Tissue Diagnostics Perspective
Applications of Rapid Immunohistochemi​stry on Frozen Tissue Sections During Intraoperative Pathological Diagnoses
RNA In Situ Hybridization:​ Applications in Anatomic Pathology
Overview of Immunohistochemi​stry Assessment of Cancer-Related Predictive Biomarkers and Common Genetic Alterations
Tissue Microarray
Unknown Primary/​Undifferentiated​ Neoplasms
Exfoliative Cytology and Effusions
Breast
Predictive Biomarkers in Breast Cancer:​ ER, PR, and HER-2/​NEU
Central Nerve System
Thyroid, Parathyroid, and Adrenal Glands
Salivary Glands and Head and Neck
Pleuropulmonary and Mediastinal Neoplasms
Uterus
Ovary
Prostate Gland
Urinary Bladder and Urachus
Kidney
Testis and Paratesticular Tissues
Pancreas and Ampulla
Liver, Bile Ducts, and Gallbladder
Upper Gastrointestinal​ Tract
Lower Gastrointestinal​ Tract and Microsatellite Instability (MSI)
Soft Tissue and Bone Tumors
Lymph Node
Bone Marrow
Cutaneous Lymphomas
Infectious Diseases
Skin
Application of Direct Immunofluorescen​ce for Skin and Mucosal Biopsies:​ A Practical Review
Application of Fluorescent In Situ Hybridization (FISH) in Surgical and Cytologic Specimens (Solid Tumors, Hematopoietic Tumors, Urine, Bile Duct Brushing, and Bronchoscopy)