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In Mass Spectrometry in Biology and Medicine, leading practitioners detail revolutionary new spectrometric techniques for the identification and covalent structural characterization of macromolecules, proteins, glycoconjugates, and nucleic acids. Based on the Fourth International Symposium on Mass Spectrometry in the Health and Life Sciences held in San Francisco in1998, this invaluable book contains tested strategies for solving many significant biomedical research problems. The techniques use mass spectrometry, automated computer processing of spectral information, and gene, protein, and EST databases for genomic and proteomic correlations.
Mass Spectrometry in Biology and Medicine offers a unique opportunity to explore and apply the new techniques of mass spectrometry that are revolutionizing the identification and structural characterization of proteins, carbohydrates, and nucleic acids. Its authoritative assessment of the field-and how to solve important and challenging problems throughout bioscience, medicine, and drug discovery-ensures a competitive advantage for today's knowledgeable researchers.
Front Matter
A New Delayed Extraction MALDI-TOF MS-MS for Characterization of Protein Digests
Measurements of Protein Structure and Noncovalent Interactions by Time-of-Flight Mass Spectrometry with Orthogonal Ion Injection
Isotopic Amplification, H/D Exchange, and Other Mass Spectrometric Strategies for Characterization of Biomacromolecular Topology and Binding Sites
Probing the Nature of Amyloidogenic Proteins by Mass Spectrometry
Studying Noncovalent Small Molecule Interactions with Protein and RNA Targets by Mass Spectrometry
Identification of Protein-Protein Interfaces by Amide Proton Exchange Coupled to MALDI-TOF Mass Spectrometry
Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation
Protein Micro-Characterization by Mass Spectrometry: Sample Handling and Data Flow
Towards an Integrated Analytical Technology for the Generation of Multidimensional Protein Expression Maps
Sequencing the Primordial Soup
Coaxial Nanospray Coupled with a Hybrid Quadrupole/Time-of-Flight Tandem Mass Spectrometer for Proteome Studies
Life Without Databases: De Novo Sequencing of Small Gene Products and Complete Characterization of Posttranslational Modifications
Investigation of Intact Subunit Polypeptide Composition of the 20S Proteasome Complex from Rat Liver Using Mass Spectrometry
Deciphering Functionally Important Multiprotein Complexes by Mass Spectrometry
Investigation of Apoptosis-Involved Processes by Mass Spectrometric Identification of the Apoptosis-Associated Proteins in IgM-Induced Burkitt Lymphoma Cells
IR-MALDI — Softer Ionization in MALDI-MS for Studies of Labile Macromolecules
Identification of in-vivo Phosphorylation Sites with Mass Spectrometry
Determination of Enzyme Mechanisms by Stopped-Flow ESI-MS
Glycosylation of Proteins — A Major Challenge in Mass Spectrometry and Proteomics
Site-specific Characterization of the N-linked Glycans of Murine PrP Sc Using Advanced Methods of Electrospray Mass Spectrometry
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of N-Linked Carbohydrates and Related Compounds
Electrophoretic and Mass Spectrometric Strategies for the Identification of Lipopolysaccharides and Immunodeterminants in Pathogenic Strains of Haemophilus influenzae  Application to Clinical Isolates
Mycobacterial Lipoglycans: Structure and Roles in Mycobacterial Immunity
The Impact of Drug Metabolism in Contemporary Drug Discovery: New Opportunities and Challenges for Mass Spectrometry
Electrospray Mass Spectrometric Analysis of Lipid Mediators Derived from Arachidonic Containing Membrane Phospholipids
Mass Spectrometry After the Human Genome Project
Problems and Prospects in the Characterization of Posttranscriptional Modifications in Large RNAs
Back Matter