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This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
Preface
Contributors
Introduction to Blood-Brain Barrier (BBB) Physiology
Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease
In Vitro Models of the Blood-Brain Barrier
Using Pluripotent Stem Cells in Models of the BBB
In Vitro Models of the Human Blood-Brain Barrier Utilising Human Induced Pluripotent Stem Cells: Opportunities and...
Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial-Brain Endothelial Cell Interactio...
An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier
A Three-Dimensional Brain-on-a-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes
Co-culture, Permeability, and Transwell Models of the BBB: Methods for Studying Shear Stress, Barrier Integrity and...
An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies
Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischemic Injury
A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier
A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress
Microfluidic Chip Models of the BBB
Novel, Emerging Chip Models of the Blood-Brain Barrier and Future Directions
Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier
Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels
BBB-on-a-Chip: Modeling Functional Human Blood-Brain Barrier by Mimicking 3D Brain Angiogenesis Using Microfluidic...
Models to Study Specific Pathologies at the BBB: Cancer, Neurodegeneration, and More
Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability
An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis
An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke
Immunohistochemical Analysis of Tight Junction Proteins
An In Vitro Blood-Brain Barrier Model to Study Firm Shear Stress-Resistant Leukocyte Adhesion to Human Brain Endot...
An In Vitro Model of the Blood–Brain Barrier to Study Alzheimer´s Disease: The Role of β-Amyloid and Its Influence...