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This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.
Production and Design
Antibiotics:​ Precious Goods in Changing Times
A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycete Culture Supernatants
Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals
Production of Antimicrobial Compounds by Homologous and Heterologous Expression
Isolation and Purification of Natural Products from Microbial Cultures
Structure Elucidation of Antibiotics
Computer-Aided Drug Design:​ An Update
Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
Mode of Action and Resistance
Microscopy-Based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action
Expansion Microscopy of Bacillus subtilis
Tracking Global and Local Changes in Membrane Fluidity Through Fluorescence Spectroscopy and Microscopy
Quantitative Analysis of Microscopy Data to Evaluate Bacterial Responses to Antibiotic Treatment
Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds
Determination of Bacterial Membrane Impairment by Antimicrobial Agents
A Colorimetric Assay to Identify and Characterize Bacterial Primase Inhibitors
Cell-Based Fluorescent Screen Amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation
Bacterial Two Component Systems:​ Overexpression and Purification:​ In Vitro and In Vivo Inhibitor Screens
Sample Preparation for Mass Spectrometry-Based Absolute Quantification of Bacterial Proteins in Antibiotic Stress Research
Elemental Analysis for the Characterization​ of Antimicrobial Effects
Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research
Functional Metagenomics to Study Antibiotic Resistance