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This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.
Saliva and Other Oral Fluids
RNA Sequencing Analysis of Saliva exRNA
Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry
Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics
Antioxidant Micronutrients and Oxidative Stress Biomarkers
Molecular Biosciences
The Oral Microbiota in Health and Disease:​ An Overview of Molecular Findings
The Long and Short of Genome Sequencing:​ Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes
Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
Bioinformatic Approaches for Describing the Oral Microbiota
Adhesion of Yeast and Bacteria to Oral Surfaces
Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)
Methods to Study Antagonistic Activities Among Oral Bacteria
Generation of Multispecies Oral Bacteria Biofilm Models
Markerless Genome Editing in Competent Streptococci
A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System
Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients
Strategy for RNA-Seq Experimental Design and Data Analysis
Characterization​ of the Expression and Role of Histone Acetylation and Deacetylation in Dental Pulp Cells
Genome-Wide Analysis of Periodontal and Peri-implant Cells and Tissues
Differential Expression, Functional and Machine Learning Analysis of High-Throughput –Omics Data Using Open-Source Tools
Micro-RNA Profiling in Dental Pulp Cell Cultures
Cells and Tissues
Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease
A Cell Culture Method for the Isolation and Study of Primary Human Dental Pulp Cells
Culturing Adipose-Derived Stem Cells Under Serum-Free Conditions
Quantitative Real-Time Gene Profiling of Human Alveolar Osteoblasts Using a One-Step System
Fabrication and Characterization​ of Decellularized Periodontal Ligament Cell Sheet Constructs
Immunohistochemi​stry and Immunofluorescen​ce
Characterization​, Quantification, and Visualization of Neutrophil Extracellular Traps
Cell Seeding on 3D Scaffolds for Tissue Engineering and Disease Modeling Applications
Workflow for Fabricating 3D-Printed Resorbable Personalized Porous Scaffolds for Orofacial Bone Regeneration
Methacrylated Gelatin as an On-Demand Injectable Vehicle for Drug Delivery in Dentistry
In Vitro Biological Testing of Dental Materials