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Optogenetics represents a breakthrough technology capable of dynamically modulating molecular and cellular activity in live cells with high precision. This transformative technology made it possible to reversibly interrogate protein actions, cellular events, and animal behaviors with a simple flash of light. Manipulating the temporal and spatial profile of light enabled precise control of membrane potentials. This feature has inspired non-opsin-based optogenetics, which not only inherits the precise spatiotemporal resolution but also expands the targets into a diverse pool of biomolecules such as membrane receptors, ion channels, kinases, GTPases, and transcription factors. This book is unique because it emphasizes the design and applications of opsin-free optogenetic tools.
Key Features
Describes new developments in non-opsin-based optogenetic tools
Introduces cutting-edge methods for precise modulation of cell signaling
Highlights applications of optogenetic regulation of cellular functions
Covers contributions from an international team of leading experts
Optogenetic Dissection of a Two-Component Calcium Infux Pathway
High-Throughput Engineering of a Light-Activatable Ca2  Channel
Optogenetic Activation of TrkB Signaling
Spatiotemporal Modulation of Neural Repair.
Optogenetic Control of Neural Stem Cell Differentiation
An Optogenetic Toolbox for Remote Control of Programmed Cell Death
Control of Protein Levels in Saccharomyces cerevisiae by Optogenetic Modules that Act on Protein Synthesis and Stability
Optogenetics as a Tool to Study Neurodegeneration and Signal Transduction
Opsin-free Optogenetics: Brain and Beyond
Constructing a Far-Red Light-Induced Split-Cre Recombinase System for Controllable Genome Engineering
Tools and Technologies for Wireless and Non-Invasive Optogenetics