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Set about five hours after the events of the film, Wesley Gibson continues his transformation into a full-fledged assassin and heir to a legacy of a secret fraternity of assassins. He continues his quest with a new mission to seek out the French chapter of the Fraternity, hunt down the Immortal, and finally discover the truth about his family.
The story begins with Wesley having a recurring bad dream that ends the same way: with a mysterious killer that murders Wesley's mother. He wakes to find that his apartment has been breached by a squadron of SWAT-like soldiers. They ransack Wesley's apartment and finally come upon a picture of his mother, Alyse. Breaking it, they find a decoded kill order in its frame. After hunting down these intruders, Wesley confronts Araña (Spanish for "Spider"), an assassin sent by the Barcelona Fraternity.
Pekwarsky (the Fraternity's master bullet-maker) arrives, manages to drive Araña off, and notifies Wesley that he's the #1 target on the Fraternity's black list. He also tells him that it will dispatch its minions to the recently ravaged Chicago Fraternity in order to collect the Loom of Fate.