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This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular techniques, protocols for isolation, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.
Preface
Contributors
An Overview of the Diversity of Bacteriophages
Structural and Genomic Diversity of Bacteriophages
The Diversity of Bacteriophages in the Human Gut
Breaking the Ice: A Review of Phages in Polar Ecosystems
The Diversity of Bacteriophages in Hot Springs
Isolation of Bacteriophages
Isolation of Bacteriophages from Soil Samples in a Poorly Equipped Field Laboratory in Kruger National Park
Purification and Up-Concentration of Bacteriophages and Viruses from Fecal Samples
Isolation of Enterococcus Bacteriophages from Municipal Wastewater Samples Using an Enrichment Step
Phage DNA Extraction, Genome Assembly, and Genome Closure
Enumeration of Bacteriophages
Enumeration of Bacteriophages by Plaque Assay
Detection and Quantification of Bacteriophages in Wastewater Samples by Culture and Molecular Methods
Flow Virometry: A Fluorescence-Based Approach to Enumerate Bacteriophages in Liquid Samples
A Metagenomics Approach to Enumerate Bacteriophages in a Food Niche
Characterization of Bacteriophages
Bioinformatic Analysis of Staphylococcus Phages: A Key Step for Safe Cocktail Development
Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus
Bacteriophage-Host Interactions and Coevolution
Unraveling Physical Interactions of Clostridioides difficile with Phage and Phage-Derived Proteins Using In Vitro...
Phage Transduction of Staphylococcus aureus
Application of Bacteriophages and Bacteriophage-Derived Components
The Next Generation of Drug Delivery: Harnessing the Power of Bacteriophages
Construction of Nonnatural Cysteine-Cross-Linked Phage Libraries
Application of Deep Sequencing in Phage Display
The Application of Bacteriophage and Photoacoustic Flow Cytometry in Bacterial Identification
Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy
Overcoming Bacteriophage Resistance in Phage Therapy
Bacteriophage Virus-Like Particles: Platforms for Vaccine Design
Index